Journal: Antioxidants & Redox Signaling

Article Title: The Neuroprotective Effect of Dimethyl Fumarate in an MPTP-Mouse Model of Parkinson's Disease: Involvement of Reactive Oxygen Species/Nuclear Factor-κB/Nuclear Transcription Factor Related to NF-E2

doi: 10.1089/ars.2016.6800

Figure Lengend Snippet: DMF treatment protects against oxidative stress. Midbrain sections were double stained for Nrf-2 (red) and NeuN (3). NeuN immunoreactivity was reduced in MPTP-injured mice (F, Q) with respect to the control group (B, Q). DMF treatment increased NeuN-positive staining at both 10 mg/kg (K, Q) and 30 mg/kg (N, K). In contrast, Nrf-2 immunoreactivity was low in the sham group (A, Q), but it increased in MPTP-injured mice (E, Q). DMF at both doses increased Nrf-2 staining (J, M, Q). Yellow spots (panels L and P) and panels D and H, are indicative of Nrf-2/NeuN co-localization. The panels (C, G, H, I and O) represent the control with DAPI to see the alive cells. Data are means ± SD of 10 mice for each group. °p < 0.05 and °°p < 0.01 versus MPTP.

Article Snippet: Sections were incubated with rabbit anti-Nrf-2 (1:100; Santa Cruz Biotechnology) or mouse monoclonal anti-neuronal nuclei (anti-NeuN) (1:100; Merck-Millipore) in a humidified chamber overnight at 37°C.

Techniques: Staining

Journal: PLoS ONE

Article Title: Retinoic Acid Restores Adult Hippocampal Neurogenesis and Reverses Spatial Memory Deficit in Vitamin A Deprived Rats

doi: 10.1371/journal.pone.0003487

Figure Lengend Snippet: Examples of : (A) Ki67-IR cells (B) DCX-IR cells and (C) 3-week-old BrdU-IR cells. (D) Three dimensional reconstruction of a z series along the y-z axis (narrow right panel) and x-z axis (narrow bottom panel) showing that a 3-week-old newly born cell (red) is double stained with the neuronal marker NeuN (green). Scale bar : A–C, 50 µm. gcl = granule cell layer.

Article Snippet: Sections were then incubated with mouse monoclonal anti-NeuN antibodies (1∶1000, Euromedex), and bound anti-NeuN monoclonal antibodies were visualized with an Alexa 488 goat anti-rabbit IgG (1∶1000, Interchim).

Techniques: Staining, Marker